关于将洋葱伯克霍尔德菌基因变种VI归为新种多洛沙伯克霍尔德菌的提议
Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov.
作者信息
Vermis Karen, Coenye Tom, LiPuma John J, Mahenthiralingam Eshwar, Nelis Hans J, Vandamme Peter
机构信息
Laboratory for Pharmaceutical Microbiology, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
Laboratory for Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
出版信息
Int J Syst Evol Microbiol. 2004 May;54(Pt 3):689-691. doi: 10.1099/ijs.0.02888-0.
Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943(T) (=CCUG 47727(T)) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.
表型和基因型研究揭示了区分洋葱伯克霍尔德菌基因变种VI与多食伯克霍尔德菌及其他洋葱伯克霍尔德菌复合群物种的新工具。因此，提议将其命名为多杀伯克霍尔德菌（Burkholderia dolosa sp. nov.），模式菌株为LMG 18943(T)（=CCUG 47727(T)）。多杀伯克霍尔德菌与其他洋葱伯克霍尔德菌复合群细菌的区别在于其不能同化色胺、壬二酸和水杨苷，且无法在洋葱伯克霍尔德菌选择性培养基PCAT上生长。16S rDNA和recA RFLP分析均显示多杀伯克霍尔德菌具有独特的限制性图谱。此外，基于新的16S rDNA和recA的PCR检测可对其进行特异性鉴定。
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