Henry D A, Mahenthiralingam E, Vandamme P, Coenye T, Speert D P
Department of Pediatrics, University of British Columbia, Vancouver, Canada.
J Clin Microbiol. 2001 Mar;39(3):1073-8. doi: 10.1128/JCM.39.3.1073-1078.2001.
Recent taxonomic advances have demonstrated that Burkholderia cepacia is a cluster of at least seven closely related genomic species (or genomovars) collectively referred to as the B. cepacia complex, all of which may cause infections among cystic fibrosis patients and other vulnerable individuals. Thus, it is important for clinical microbiologists to be able to differentiate genomovars. Prior to this study, 361 B. cepacia complex isolates and 51 isolates easily confused with B. cepacia complex previously had been identified using a polyphasic approach, and in this study, a comparison of phenotypic and biochemical tests was carried out. It was determined that Burkholderia multivorans and Burkholderia stabilis could reliably be separated from other members of the B. cepacia complex by phenotypic methods. A combination of phenotypic and molecular tests such as recA PCR and 16S rRNA RFLP are recommended for differentiation among the genomovars of the B. cepacia complex. A biochemical reaction scheme for the identification of B. gladioli, Pandoraea species, and Ralstonia pickettii and the differentiation of these species from the B. cepacia complex is also presented.
最近的分类学进展表明，洋葱伯克霍尔德菌是至少七个密切相关的基因组种（或基因组变种）的一个集群，统称为洋葱伯克霍尔德菌复合体，所有这些都可能在囊性纤维化患者和其他易感个体中引起感染。因此，临床微生物学家能够区分基因组变种很重要。在本研究之前，已经使用多相方法鉴定了361株洋葱伯克霍尔德菌复合体分离株和51株以前容易与洋葱伯克霍尔德菌复合体混淆的分离株，并且在本研究中，进行了表型和生化试验的比较。结果确定，多食伯克霍尔德菌和稳定伯克霍尔德菌可以通过表型方法可靠地与洋葱伯克霍尔德菌复合体的其他成员区分开来。建议结合表型和分子试验，如recA PCR和16S rRNA RFLP，来区分洋葱伯克霍尔德菌复合体的基因组变种。还介绍了一种用于鉴定唐菖蒲伯克霍尔德菌、潘多拉菌属物种和皮氏罗尔斯通菌以及将这些物种与洋葱伯克霍尔德菌复合体区分开来的生化反应方案。
