Detection of bone marrow infiltration of lymphoma cells by fluorescence in situ hybridization.
作者信息
Ishizawa Kenichi, Obara Yasuhiko, Seki Osamu, Fujimaki Shinichi, Kameoka Junichi, Ichinohasama Ryo, Kanemitsu Keiji, Sasaki Takeshi, Kaku Mitsuo, Harigae Hideo
机构信息
Department of Rheumatology and Hematology, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
出版信息
Clin Chim Acta. 2004 Jun;344(1-2):79-82. doi: 10.1016/j.cccn.2004.02.014.
BACKGROUND
It is sometimes difficult to detect the bone marrow infiltration of lymphoma cells, because lymphoma cells are not distinguishable from normal lymphocytes due to the similarity of their phenotype.
METHODS
Bone marrow involvement of 17 samples of 15 patients with follicular lymphoma, whose lymphoma cells were confirmed to harbor the translocation of chromosome14q32, were examined by microscopic analysis of bone marrow smear and biopsy, flow cytometorical analysis (FCM), chromosomal analysis of G-banding and fluorescence in situ hybridization (FISH). FISH was performed using a probe, which detects the split of IGH gene on 14q32.
RESULTS
The positivity of FISH was highest among these methods and FISH was able to detect the bone marrow involvement in one case who was defined as negative by bone marrow biopsy.
CONCLUSIONS
FISH can be used for detection of bone marrow involvement of malignant lymphoma that carries chromosomal rearrangement involving 14q32.
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