Hung C R, Takeuchi K, Okabe S, Murata T, Takagi K
Jpn J Pharmacol. 1976 Dec;26(6):703-9. doi: 10.1254/jjp.26.703.
Effects of L-glutamine on acetylsalicylic acid (ASA)-induced gastric mucosal lesions were studied in mongrel dogs. It was confirmed that when oral ASA at 1.0 or 2.0 g per dog is given in two divided doses, there is severe and consistent dose-dependent mucosal damage in the glandular portion of the stomach in fasted dogs. However, when L-glutamine 2.0 or 4.0 g per dog in two divided doses is given concomitantly with ASA 2.0 g per dog orally, the gastric irritation is significantly inhibited. Instillation of 20 mM of ASA in 100 mM HCl solution into the Heidenhain pouch of Beagle dogs produced a significant loss of H+ from the pouch and a gain of Na+ in the lumen compared with ASA-free controls. When L-glutamine (100 mM) was given concomitantly with ASA (20 mM) into the pouch, changes of electrolyte fluxes in response to ASA alone were significantly suppressed. However, 50 mM of L-glutamine had no appreciable effect on acid back diffusion caused by ASA 20 mM. The amino acid itself had little effect on the ionic movement in the pouch. Gross bleeding from the pouch treated with ASA was never observed with the concomitant dosing of ASA and L-glutamine 50 or 100 mM.
在杂种犬中研究了L-谷氨酰胺对乙酰水杨酸（ASA）诱导的胃黏膜损伤的影响。已证实，当每只犬口服1.0或2.0 g ASA并分两次给药时，禁食犬胃腺部会出现严重且一致的剂量依赖性黏膜损伤。然而，当每只犬同时口服2.0或4.0 g L-谷氨酰胺并分两次给药，且同时口服每只犬2.0 g ASA时，胃刺激会受到显著抑制。与无ASA的对照组相比，将含20 mM ASA的100 mM HCl溶液注入比格犬的海登海因小胃，会导致小胃中H⁺显著丢失，管腔中Na⁺增加。当将L-谷氨酰胺（100 mM）与ASA（20 mM）同时注入小胃时，单独使用ASA引起的电解质通量变化会受到显著抑制。然而，50 mM的L-谷氨酰胺对20 mM ASA引起的酸反向扩散没有明显影响。氨基酸本身对小胃中的离子运动影响很小。在同时给予ASA和50或100 mM L-谷氨酰胺时，从未观察到用ASA处理的小胃出现明显出血。
