来自乳酸克鲁维酵母的两个编码谷胱甘肽还原酶和硫氧还蛋白还原酶的核基因的分离与鉴定。

Isolation and characterization of two nuclear genes encoding glutathione and thioredoxin reductases from the yeast Kluyveromyces lactis.

作者信息

Tarrío N, Díaz Prado S, Cerdán M E, González Siso M I

机构信息

Department of Molecular and Cell Biology, University of A Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain.

出版信息

Biochim Biophys Acta. 2004 May 25;1678(2-3):170-5. doi: 10.1016/j.bbaexp.2004.03.004.

DOI:10.1016/j.bbaexp.2004.03.004

PMID:15157744

Abstract

Response to oxidative stress has been hitherto scarcely studied in the respiratory yeast Kluyveromyces lactis. The genes coding for reductases of glutathione and thioredoxin, KlGLR1 and KlTRR1, respectively, have been cloned and characterized in this work. H(2)O(2) treatment increased transcription and enzyme activity of KlTRR1 but not of KlGLR1, suggesting a different situation from that reported for the fermentative yeast Saccharomyces cerevisiae. A consensus for Yap1p binding is functional in the KlTRR1 promoter.

摘要

迄今为止，在呼吸型酵母乳酸克鲁维酵母中，对氧化应激的反应鲜有研究。在本研究中，分别克隆并鉴定了编码谷胱甘肽还原酶和硫氧还蛋白还原酶的基因KlGLR1和KlTRR1。H₂O₂处理增加了KlTRR1的转录和酶活性，但未增加KlGLR1的转录和酶活性，这表明与发酵型酵母酿酒酵母的情况不同。Yap1p结合的共有序列在KlTRR1启动子中起作用。