硫氧还蛋白还原酶对于真菌病原体新型隐球菌的生存能力至关重要。
Thioredoxin reductase is essential for viability in the fungal pathogen Cryptococcus neoformans.
作者信息
Missall Tricia A, Lodge Jennifer K
机构信息
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO 63104, USA.
出版信息
Eukaryot Cell. 2005 Feb;4(2):487-9. doi: 10.1128/EC.4.2.487-489.2005.
Abstract
Thioredoxin reductase (TRR1) is an important component of the thioredoxin oxidative stress resistance pathway. Here we show that it is induced during oxidative and nitrosative stress and is preferentially localized to the mitochondria in Cryptococcus neoformans. The C. neoformans TRR1 gene encodes the low-molecular-weight isoform of the thioredoxin reductase enzyme, which shares little homology with that of its mammalian host. By replacing the endogenous TRR1 promoter with an inducible copper transporter promoter, we showed that Trr1 appears to be essential for viability of this pathogenic fungus, making it a potential antifungal target.
摘要
硫氧还蛋白还原酶（TRR1）是硫氧还蛋白抗氧化应激途径的重要组成部分。在此我们表明，它在氧化应激和亚硝化应激期间被诱导，并且在新型隐球菌中优先定位于线粒体。新型隐球菌TRR1基因编码硫氧还蛋白还原酶的低分子量异构体，它与其哺乳动物宿主的该酶几乎没有同源性。通过用可诱导的铜转运蛋白启动子替代内源性TRR1启动子，我们表明Trr1似乎对这种致病真菌的生存能力至关重要，使其成为潜在的抗真菌靶点。
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