从食用菌巨大口蘑的新鲜子实体中分离抗真菌蛋白木霉菌素。

Isolation of trichogin, an antifungal protein from fresh fruiting bodies of the edible mushroom Tricholoma giganteum.

作者信息

Guo Yongxia, Wang Hexiang, Ng T B

机构信息

Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.

出版信息

Peptides. 2005 Apr;26(4):575-80. doi: 10.1016/j.peptides.2004.11.009.

DOI:10.1016/j.peptides.2004.11.009

PMID:15752570

Abstract

An antifungal protein was isolated from the mushroom Tricholoma giganteum var. golden blessings. The protocol included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal protein, designated trichogin, was unadsorbed on DEAE-cellulose but was adsorbed on Affi-gel blue gel and CM-cellulose. It exhibited antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. Trichogin inhibited HIV-1 reverse transcriptase with an IC50 of 83 nM.

摘要

从巨大口蘑变种“金色祝福”中分离出一种抗真菌蛋白。该实验方案包括在DEAE - 纤维素上进行离子交换色谱、在Affi - 凝胶蓝胶上进行亲和色谱、在CM - 纤维素上进行离子交换色谱以及在Superdex 75上通过快速蛋白质液相色谱进行凝胶过滤。这种抗真菌蛋白被命名为口蘑素，它不被DEAE - 纤维素吸附，但能被Affi - 凝胶蓝胶和CM - 纤维素吸附。它对尖孢镰刀菌、花生球腔菌和梨轮纹病菌表现出抗真菌活性。口蘑素抑制HIV - 1逆转录酶的IC50为83 nM。