侧耳菌素，一种来自平菇的抗真菌肽。

Pleurostrin, an antifungal peptide from the oyster mushroom.

作者信息

Chu K T, Xia Lixin, Ng T B

机构信息

Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

出版信息

Peptides. 2005 Nov;26(11):2098-103. doi: 10.1016/j.peptides.2005.04.010. Epub 2005 Jun 6.

DOI:10.1016/j.peptides.2005.04.010

PMID:15941607

Abstract

A 7kDa peptide, with inhibitory activity on mycelial growth in the fungi Fusaerium oxysporum, Mycosphaerella arachidicola and Physalospora piricola, was isolated from fresh fruiting bodies of the oyster mushroom. The isolation procedure entailed extraction with an aqueous buffer, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel and gel filtration by fast protein liquid chromatography on Superdex 75. The protein was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel. It demonstrated an N-terminal sequence different from known antifungal proteins and peptides.

摘要

从平菇新鲜子实体中分离出一种7千道尔顿的肽，它对尖孢镰刀菌、花生球腔菌和梨孢盘菌的菌丝体生长具有抑制活性。分离过程包括用水性缓冲液提取、在DEAE - 纤维素上进行离子交换色谱、在Affi - gel蓝凝胶上进行亲和色谱以及在Superdex 75上通过快速蛋白质液相色谱进行凝胶过滤。该蛋白质不吸附于DEAE - 纤维素，而吸附于Affi - gel蓝凝胶。它显示出与已知抗真菌蛋白质和肽不同的N端序列。