乳胶凝集试验、湿片法及培养法检测阴道毛滴虫的比较
Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis.
作者信息
Adu-Sarkodie Y, Opoku B K, Danso K A, Weiss H A, Mabey D
机构信息
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
出版信息
Sex Transm Infect. 2004 Jun;80(3):201-3. doi: 10.1136/sti.2003.007864.
OBJECTIVES
To compare the performance of three diagnostic methods for Trichomonas vaginalis infection-latex agglutination, saline wet mount, and culture.
METHODS
Vaginal swabs from 3807 women attending antenatal clinics were tested for the presence of T vaginalis by latex agglutination. All positives and the following two negatives were tested by wet preparation and culture.
RESULTS
The prevalence of infection by latex agglutination was 5.4%. Using an expanded gold standard based on the wet mount and culture results, the sensitivity of the latex agglutination test was 98.8% (95% CI 95.9 to 99.9) and specificity was 92.1 (89.2 to 94.5). The kappa index for test agreement was 0.93 for latex and culture and 0.88 for latex and wet preparation.
CONCLUSION
The latex agglutination test is a highly sensitive test for detecting T vaginalis infection. It is a simple rapid test and has the potential for use in screening and diagnostic settings.
目的
比较三种诊断阴道毛滴虫感染的方法——乳胶凝集试验、生理盐水湿片法和培养法的性能。
方法
对3807名到产前门诊就诊的女性的阴道拭子进行乳胶凝集试验，检测是否存在阴道毛滴虫。所有阳性样本以及接下来的两个阴性样本进行湿片法和培养法检测。
结果
乳胶凝集试验检测出的感染率为5.4%。基于湿片法和培养法结果建立的扩展金标准显示，乳胶凝集试验的灵敏度为98.8%（95%可信区间为95.9至99.9），特异性为92.1（89.2至94.5）。乳胶凝集试验与培养法的试验一致性kappa指数为0.93，乳胶凝集试验与湿片法的kappa指数为0.88。
结论
乳胶凝集试验是检测阴道毛滴虫感染的高灵敏度试验。它是一种简单快速的试验，有用于筛查和诊断的潜力。
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