Sasagawa S, Satow Y, Suzuki K, Hosokawa T
Radiation Effects Research Foundation, Hiroshima, Japan.
Immunopharmacol Immunotoxicol. 1992;14(3):625-35. doi: 10.3109/08923979209005414.
Chemotactic responsiveness to fMet-Leu-Phe in concentrations of 10(-8) to 10(-4) M in the Boyden chamber was compared between peritoneal exudate cells (PEC) and polymorphonuclear leukocytes (PMN) isolated from peripheral blood, between MRL/Mp-+/+ (MRL-+/+) and MRL/Mp-lpr/lpr (MRL-lpr/lpr) mice, and between young (6-9 week old) and aged (16-24 week old) mice. Chemotactic responsiveness of PEC did not differ between MRL-+/+ and MRL-lpr/lpr, and young and aged mice. While, PMN showed greater chemotaxis in aged MRL-+/+ mice than that in aged MRL-lpr/lpr mice. These results suggest that chemotactic responsiveness of PMN differ from that of PEC which is assumed to be preactivated by an inflammatory agent injected into the peritoneal cavity to elicit cells. Less responsiveness of PMN to the bacterial origin peptide might relate to the autoimmune disease of this murine model.
比较了从外周血分离的腹腔渗出细胞（PEC）和多形核白细胞（PMN）、MRL/Mp-+/+（MRL-+/+）和MRL/Mp-lpr/lpr（MRL-lpr/lpr）小鼠以及年轻（6-9周龄）和老年（16-24周龄）小鼠对浓度为10^(-8)至10^(-4)M的fMet-Leu-Phe在Boyden小室中的趋化反应性。PEC的趋化反应性在MRL-+/+和MRL-lpr/lpr以及年轻和老年小鼠之间没有差异。然而，老年MRL-+/+小鼠中的PMN比老年MRL-lpr/lpr小鼠中的PMN表现出更大的趋化性。这些结果表明，PMN的趋化反应性与PEC不同，PEC被认为是由注入腹腔以引出细胞的炎症剂预激活的。PMN对细菌来源肽的反应性较低可能与该小鼠模型的自身免疫疾病有关。
