Yu C, Sun K, Tsai C, Tsai Y, Tsai S, Huang D, Han S, Yu H
Department of Medicine, Veterans General Hospital-Taipei, Taiwan, China.
Immunology. 1998 Nov;95(3):480-7. doi: 10.1046/j.1365-2567.1998.00624.x.
Peritoneal exudative polymorphonuclear neutrophils (PEC-PMN) and mononuclear cells (PEC-MNC) were obtained from normal BALB/c and from autoimmune MRL-lpr/lpr mice (lpr) with different disease severities. The spontaneous and mitogen-stimulated expression of T-helper lymphocyte type-1 (Th1) [represented by interferon-gamma (IFN-gamma) and interleukin (IL-2)] and T-helper lymphocyte type-2 (Th2) (represented by IL-4 and IL-10) cytokine mRNA in these cells was detected by reverse transcription-polymerase chain reaction (RT-PCR). The production of these cytokines was measured by enzyme-linked immunosorbent assay (ELISA). We found that the spontaneous expression of Th1/Th2 cytokine mRNA in PEC-PMN from autoimmune mice was progressively increased in parallel with disease severity but was not changed by lipopolysaccharide (LPS) stimulation. By contrast, spontaneous expression of Th1/Th2 cytokine mRNA in PEC-MNC from these mice was progressively decreased in parallel with disease severity but retained the responsiveness to phytohaemagglutinin (PHA) stimulation. To determine the effect of PEC-PMN on Th1/Th2 cytokine production by PEC-MNC, autologous PEC-PMN and PEC-MNC were co-cultured at MNC:PMN ratios of 5:0, 4:1, 3:2, 2:3, 1:4 and 0:5 with PHA stimulation for 24 hr. The production of cytokines at each ratio was compared with the expected value, by calculation. We found that PEC-PMN from autoimmune mice progressively suppressed the production of IL-4, IL-10 and IFN-gamma whereas the production of IL-2 was enhanced by autologous MNC in parallel with disease severity. These results suggest that a reciprocal relationship exists in the expression of Th1/Th2 cytokine mRNA between PEC-PMN and PEC-MNC in lpr mice in parallel with disease severity. Autoimmune PEC-PMN can exert significant modulatory effects on Th1/Th2 cytokine production by autologous MNC in stimulation.
从正常BALB/c小鼠以及患有不同疾病严重程度的自身免疫性MRL-lpr/lpr小鼠（lpr）中获取腹腔渗出多形核中性粒细胞（PEC-PMN）和单核细胞（PEC-MNC）。通过逆转录-聚合酶链反应（RT-PCR）检测这些细胞中1型辅助性T淋巴细胞（Th1）[以干扰素-γ（IFN-γ）和白细胞介素（IL-2）为代表]和2型辅助性T淋巴细胞（Th2）（以IL-4和IL-10为代表）细胞因子mRNA的自发表达及丝裂原刺激后的表达。通过酶联免疫吸附测定（ELISA）测量这些细胞因子的产生。我们发现，来自自身免疫小鼠的PEC-PMN中Th1/Th2细胞因子mRNA的自发表达随着疾病严重程度的增加而逐渐升高，但脂多糖（LPS）刺激对其无影响。相比之下，这些小鼠的PEC-MNC中Th1/Th2细胞因子mRNA的自发表达随着疾病严重程度的增加而逐渐降低，但对植物血凝素（PHA）刺激仍保持反应性。为了确定PEC-PMN对PEC-MNC产生Th1/Th2细胞因子的影响，将自体PEC-PMN和PEC-MNC以MNC:PMN为5:0、4:1、3:2、2:3、1:4和0:5的比例共同培养，并用PHA刺激24小时。通过计算将每个比例下细胞因子的产生与预期值进行比较。我们发现，来自自身免疫小鼠的PEC-PMN逐渐抑制IL-4、IL-10和IFN-γ的产生，而IL-2的产生则随着疾病严重程度的增加被自体MNC增强。这些结果表明，在lpr小鼠中，PEC-PMN和PEC-MNC之间Th1/Th2细胞因子mRNA的表达与疾病严重程度呈平行关系，存在相互作用。自身免疫性PEC-PMN在刺激下可对自体MNC产生Th1/Th2细胞因子发挥显著的调节作用。
