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含蛋白质的双水相体系粘度评估。

Evaluation of viscosities of aqueous two-phase systems containing protein.

作者信息

Gündüz Ufuk

机构信息

Kimya Mühendisliği Bölümü, Mühendislik ve Mimarlik Fakültesi, Gazi Universitesi, 06570 Ankara, Turkey.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jul 25;807(1):157-61. doi: 10.1016/j.jchromb.2004.01.048.

Abstract

The dynamic viscosities of aqueous polyethylene glycol, aqueous bovine serum albumin, and polyethylene glycol-bovine serum albumin-water solutions were measured at temperatures of 15, 20, 25, 30 and 35 degree C. To estimate the viscosity values of polyethylene glycol-bovine serum albumin-water solutions, a one parameter Grunberg-like model which was satisfactorily used earlier by the present author for polyethylene glycol-dextran-water solutions was employed. The disposable parameter a for our temperature range was estimated as 3.71. The relative errors varying from 0.29 to 18.98 in absolute value indicates that the Grunberg-like model works perfectly for polymer-protein solutions as well.

摘要

在15、20、25、30和35摄氏度的温度下,测量了聚乙二醇水溶液、牛血清白蛋白水溶液以及聚乙二醇 - 牛血清白蛋白 - 水溶液的动态粘度。为了估算聚乙二醇 - 牛血清白蛋白 - 水溶液的粘度值,采用了一个单参数类格伦贝格模型,作者此前曾成功地将该模型用于聚乙二醇 - 葡聚糖 - 水溶液。我们所研究温度范围内的一次性参数a估计为3.71。绝对值在0.29至18.98之间变化的相对误差表明,类格伦贝格模型对于聚合物 - 蛋白质溶液同样适用。

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