Chethana S, Rastogi N K, Raghavarao K S M S
Department of Food Engineering, Central Food Technological Research Institute, 570 020, Mysore, India.
Biotechnol Lett. 2006 Jan;28(1):25-8. doi: 10.1007/s10529-005-4682-5.
A new aqueous two phase system comprising polyethylene glycol and xanthan is reported together with its phase diagram. The phase composition of the bottom phase did not vary (PEG 1.6-1.8% w/w; xanthan 0.24-0.28% w/w) while that of the top phase varied significantly (PEG 4-5% w/w, xanthan 0.05-1.37% w/w). Unlike conventional aqueous two phase systems, the viscosity of the top phase is also high and values are comparable to that of the bottom phase. When BSA was used as a model protein, it partitioned entirely into the bottom phase.
报道了一种由聚乙二醇和黄原胶组成的新型双水相体系及其相图。下层相的相组成没有变化（聚乙二醇1.6 - 1.8% w/w；黄原胶0.24 - 0.28% w/w），而上层相的相组成变化显著（聚乙二醇4 - 5% w/w，黄原胶0.05 - 1.37% w/w）。与传统双水相体系不同，上层相的粘度也很高，其值与下层相相当。当使用牛血清白蛋白作为模型蛋白时，它完全分配到下层相中。
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