特定微生物来源的果聚糖多糖的抗肿瘤活性

Antitumor activity of levan polysaccharides from selected microorganisms.

作者信息

Yoo Sang-Ho, Yoon Eun Ju, Cha Jaeho, Lee Hyeon Gyu

机构信息

Department of Food Science and Technology, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul 143-747, South Korea.

出版信息

Int J Biol Macromol. 2004 Apr;34(1-2):37-41. doi: 10.1016/j.ijbiomac.2004.01.002.

DOI:10.1016/j.ijbiomac.2004.01.002

PMID:15178007

Abstract

Levans were isolated from the cultures of Gluconoacetobacter xylinus (G-levan; Mw = 40,000), Microbacterium laevaniformans (M; Mw = 710,000), Rahnella aquatilis (R; Mw = 380,000), and Zymomonas mobilis (Z; Mw = 570,000). The levans were composed mainly of fructose residues when analyzed by TLC and HPLC, and their main backbones were beta-(2,6)-linkages with beta-(2,1)-branches by GC-MS and NMR. In the in vitro antitumor activity test of the levans against eight different tumor cell lines, relatively stronger activity was observed from the SNU-1 and HepG2. The M- (52.54-62.05%) and R-levan (52.15-58.58%) showed the significantly high activity against SNU-1, while M-levan showed the highest (49.93-61.82%) activity against HepG2. During the in vivo analysis of inhibitory activity of the levans against Sarcoma-180 growth, M-, R- and Z-levans showed strong antitumor activity (average 66%) but G-levan (42%) had significantly lower activity.

摘要

果聚糖是从木醋杆菌（G - 果聚糖；Mw = 40,000）、类果聚糖微杆菌（M；Mw = 710,000）、水生拉恩菌（R；Mw = 380,000）和运动发酵单胞菌（Z；Mw = 570,000）的培养物中分离得到的。通过薄层色谱法（TLC）和高效液相色谱法（HPLC）分析，这些果聚糖主要由果糖残基组成，通过气相色谱 - 质谱联用仪（GC - MS）和核磁共振（NMR）分析，其主要骨架为带有β-(2,1)分支的β-(2,6)连接。在果聚糖对八种不同肿瘤细胞系的体外抗肿瘤活性测试中，在SNU - 1和HepG2细胞系中观察到相对较强的活性。M - 果聚糖（52.54 - 62.05%）和R - 果聚糖（52.15 - 58.58%）对SNU - 1显示出显著的高活性，而M - 果聚糖对HepG2显示出最高活性（49.93 - 61.82%）。在果聚糖对肉瘤180生长的体内抑制活性分析中，M - 、R - 和Z - 果聚糖显示出较强的抗肿瘤活性（平均66%），但G - 果聚糖（42%）的活性明显较低。