Haddar Anissa, Ben Ayed Emna, Sila Assaad, Putaux Jean-Luc, Bougatef Ali, Boufi Sami
Laboratory of Plants Improvement and Valorization of Agroressources (LAPVA), National School of Engineering of Sfax (ENIS), University of Sfax 3038 Sfax Tunisia
University of Sfax, High Institute of Biotechnology Sfax Tunisia.
RSC Adv. 2021 Dec 6;11(62):38990-39003. doi: 10.1039/d1ra07852f.
Foodborne diseases caused by resistance of microorganisms to multiple antimicrobial agents have emerged as a major public health concern around the world. The search for potential antimicrobials has resulted in the emergence of metal nanoparticles for protection against these infections. In this study an eco-friendly and green approach was used to biosynthesize hybrid Ag/AgCl nanoparticles (NPs), using levan from as a stabilizing/reducing agent, with a high efficiency against a broad spectrum of foodborne bacteria as well as biofilm formations. The morphology and physicochemical characteristics of levan-Ag/AgCl NPs were investigated by transmission electron microscopy (TEM), X-ray diffraction (XRD), UV-vis spectroscopy (UV), dynamic light scattering (DLS) and thermogravimetric analysis (TGA). The hybrid levan-Ag/AgCl was evaluated for antibacterial activity against foodborne pathogenic bacteria (, , , , , , , , and ). The study demonstrated the strong efficiency of hybrid levan-Ag/AgCl NPs as a potent inhibitor against all tested strains, with much higher activity against Gram-negative than Gram-positive bacteria. Furthermore, bacterial strains were found to be highly sensitive to hybrid levan-Ag/AgCl NPs in comparison to the tested antibiotics. As a possible application of levan-Ag/AgCl NPs as an additive in packaging, PVA films with different amounts of hybrid levan-Ag/AgCl NPs were prepared by casting and their antibacterial, mechanical, and optical properties and ability to expand the shelf life of beef meat were explored. Interestingly, the amount of Ag leached out from films was below the permissible limit. This work demonstrates the strong antibacterial action of hybrid levan-Ag/AgCl NPs and their potential use in bioactive packaging material.
微生物对多种抗菌剂产生耐药性所引发的食源性疾病已成为全球主要的公共卫生问题。对潜在抗菌剂的探索促使金属纳米颗粒出现，用于预防这些感染。在本研究中，采用了一种环保的绿色方法，以果聚糖作为稳定/还原剂，生物合成了具有高效抗菌活性的杂化Ag/AgCl纳米颗粒（NPs），该纳米颗粒对多种食源性细菌以及生物膜形成均具有高效抑制作用。通过透射电子显微镜（TEM）、X射线衍射（XRD）、紫外可见光谱（UV）、动态光散射（DLS）和热重分析（TGA）对果聚糖-Ag/AgCl NPs的形态和理化特性进行了研究。评估了杂化果聚糖-Ag/AgCl对食源性病原体细菌（、、、、、、、、和）的抗菌活性。该研究表明杂化果聚糖-Ag/AgCl NPs作为一种有效的抑制剂，对所有测试菌株均具有很强的抑制效果，对革兰氏阴性菌的活性远高于革兰氏阳性菌。此外，与测试的抗生素相比，发现细菌菌株对杂化果聚糖-Ag/AgCl NPs高度敏感。作为果聚糖-Ag/AgCl NPs在包装中作为添加剂的一种可能应用，通过流延法制备了含有不同量杂化果聚糖-Ag/AgCl NPs的聚乙烯醇（PVA）薄膜，并探索了其抗菌、机械、光学性能以及延长牛肉保质期的能力。有趣的是，从薄膜中浸出的银量低于允许限度。这项工作证明了杂化果聚糖-Ag/AgCl NPs具有很强的抗菌作用及其在生物活性包装材料中的潜在用途。
