Zhang Zhenqing, Yu Guangli, Guan Huashi, Zhao Xia, Du Yuguo, Jiang Xiaolu
Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, China.
Carbohydr Res. 2004 Jun 1;339(8):1475-81. doi: 10.1016/j.carres.2004.03.010.
Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.
在特定反应条件下，从海洋细菌弧菌属510的发酵液中纯化得到的藻酸盐，用藻酸裂解酶进行水解。通过低压凝胶渗透色谱法（LP-GPC）和半制备型强阴离子交换（SAX）快速蛋白质液相色谱法（FPLC）分离出包括二糖、三糖和四糖在内的七种寡糖。基于电喷雾离子化质谱（ESIMS）和二维核磁共振（2D NMR）光谱分析阐明了寡糖的结构。讨论了这种藻酸裂解酶对藻酸盐的水解特异性。
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