由假单胞菌 HZJ 216 来源的海藻酸钠裂解酶降解制备、纯化和表征海藻酸钠寡糖。
Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216.
机构信息
School of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, PR China.
出版信息
Carbohydr Res. 2011 May 1;346(6):794-800. doi: 10.1016/j.carres.2011.01.023. Epub 2011 Jan 28.
PMID:21371694
Abstract
Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.
摘要
从海洋细菌假单胞菌 HJZ216 的发酵液中纯化得到的褐藻胶裂解酶被用于水解藻类褐藻胶。通过阴离子交换色谱分离和纯化了 6 种寡糖，包括二糖和三糖。根据电喷雾电离质谱（ESI-MS）和二维 NMR 谱分析确定了寡糖的结构。
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