Laboratory of Food Chemistry, Wageningen University, Wageningen, The Netherlands.
J Agric Food Chem. 2013 Jan 23;61(3):553-60. doi: 10.1021/jf304338z. Epub 2013 Jan 10.
This research aimed to develop a method for analyzing specific alginate oligosaccharides (AOS) in a complex matrix such as pig feces. The data obtained were used to study alginate degradation by the microbiota in the large intestine during adaptation, including the individual variation between pigs. A method using an UHPLC system with an ethylene bridged hybrid (BEH) amide column coupled with MS(n) detection was able to distinguish saturated and unsaturated AOS with DP 2-10. Isomers of unsaturated trimer and tetramer could be separated and annotated. In the feces, saturated and unsaturated AOS were present. The presence of unsaturated AOS indicates that the microbiota produced alginate lyase. The microbiota utilized unsaturated AOS more than saturated AOS. The results also suggested that guluronic acid at the reducing end of AOS inhibits the utilization by microbiota during the first weeks of adaptation. After adaptation, the microbiota was able to utilize a broader range of AOS.
本研究旨在开发一种分析复杂基质（如猪粪便）中特定褐藻糖胶寡糖（AOS）的方法。所获得的数据用于研究在适应过程中大肠微生物群对褐藻胶的降解，包括猪之间的个体差异。一种使用带有 MS(n) 检测的超高效液相色谱系统和乙烯桥杂化（BEH）酰胺柱的方法能够区分 DP 2-10 的饱和和不饱和 AOS。不饱和三聚体和四聚体的异构体可以分离和注释。在粪便中存在饱和和不饱和 AOS。不饱和 AOS 的存在表明微生物群产生了褐藻胶裂解酶。微生物群利用不饱和 AOS 多于饱和 AOS。结果还表明，AOS 还原端的古洛糖醛酸在适应的前几周会抑制微生物群的利用。适应后，微生物群能够利用更广泛范围的 AOS。
