使用DOGMA对细胞器基因组进行自动注释。

Automatic annotation of organellar genomes with DOGMA.

作者信息

Wyman Stacia K, Jansen Robert K, Boore Jeffrey L

机构信息

Department of Computer Sciences, Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.

出版信息

Bioinformatics. 2004 Nov 22;20(17):3252-5. doi: 10.1093/bioinformatics/bth352. Epub 2004 Jun 4.

DOI:10.1093/bioinformatics/bth352

PMID:15180927

Abstract

The Dual Organellar GenoMe Annotator (DOGMA) automates the annotation of organellar (plant chloroplast and animal mitochondrial) genomes. It is a Web-based package that allows the use of BLAST searches against a custom database, and conservation of basepairing in the secondary structure of animal mitochondrial tRNAs to identify and annotate genes. DOGMA provides a graphical user interface for viewing and editing annotations. Annotations are stored on our password-protected server to enable repeated sessions of working on the same genome. Finished annotations can be extracted for direct submission to GenBank.

摘要

双细胞器基因组注释器（DOGMA）可自动注释细胞器（植物叶绿体和动物线粒体）基因组。它是一个基于网络的软件包，允许针对自定义数据库使用BLAST搜索，并利用动物线粒体tRNA二级结构中的碱基配对保守性来识别和注释基因。DOGMA提供了一个图形用户界面用于查看和编辑注释。注释存储在我们受密码保护的服务器上，以便能够对同一基因组进行重复的注释工作会话。完成的注释可以提取出来直接提交到GenBank。