Sagawa K
Department of Immunology, Kurume University School of Medicine.
Nihon Rinsho. 1992 Jun;50(6):1209-16.
Clinically useful monoclonal antibodies, applied for immunophenotyping of leukemias, are reviewed. With a combination of 15 antibodies, including CD2, CD3, CD4, CD5, CD7, and CD8 for T cell marker analysis, CD10, CD19, CD20, surface immunoglobulins, and cytoplasmic mu chain for B cell marker analysis, CD13 and CD33 for myeloid marker analysis, and HLA-DR and CD25 for other marker analysis, acute lymphoblastic leukemias of T cell type, cALL type, pre-B cell type and B cell type, acute myeloid leukemias, acute unclassified leukemias and adult T cell leukemias could be clearly diagnosed by immunophenotyping of cell membrane molecules. By using additional CD11b, CD14, and CD15 monoclonal antibodies, subclassification of acute myeloid leukemia was partially possible.
本文综述了用于白血病免疫表型分析的临床实用单克隆抗体。通过组合15种抗体，包括用于T细胞标志物分析的CD2、CD3、CD4、CD5、CD7和CD8，用于B细胞标志物分析的CD10、CD19、CD20、表面免疫球蛋白和细胞质μ链，用于髓系标志物分析的CD13和CD33，以及用于其他标志物分析的HLA - DR和CD25，可通过细胞膜分子的免疫表型分析清晰诊断T细胞型、普通型急性淋巴细胞白血病（cALL）、前B细胞型和B细胞型急性淋巴细胞白血病、急性髓系白血病、急性未分类白血病和成人T细胞白血病。通过使用额外的CD11b、CD14和CD15单克隆抗体，急性髓系白血病的亚分类部分可行。
