Dimitrijević H, Kamenov B, Milenović D, Kostić G, Vojinović J
Srp Arh Celok Lek. 1994;122 Suppl 1:108-10.
Markers of differentiation on cell membrane are recognized by monoclonal antibodies, capable to define more precisely the level of cell maturation. The immunofluorescent method, used in this work, is based on the discoveration of surface molecules by means of fluorescein labeled monoclonal antibodies. 74 patients with leukemia, 11 children and 63 adults persons, have been examined. The results show that malignant cells express certain markers characteristic for some forms of leukemias. The most common marker expression in pre-B ALL was: CD34, CD10, CD22, CD24, CD19 and DR. Expression of CD10 marker was characteristic for CALLA + pre-B ALL cells. T leukemias expressed CD38, CD2, CD3, CD4 and CD8 markers. The expression of CD36, CD13, CD14, CD15, CD11b and DR markers was seen in AML. Surface markers may determine type of leikemia. Immunophenotyping of leukemias has enabled us to make a precise diagnostic, determine its prognosis, and select appropriate therapeutic approach.
细胞膜上的分化标志物可被单克隆抗体识别，这些抗体能够更精确地界定细胞成熟水平。本研究中使用的免疫荧光法，是基于通过荧光素标记的单克隆抗体来发现表面分子。已对74例白血病患者进行了检查，其中包括11名儿童和63名成人。结果表明，恶性细胞表达某些特定于某些白血病形式的标志物。前B淋巴细胞白血病最常见的标志物表达为：CD34、CD10、CD22、CD24、CD19和DR。CD10标志物的表达是普通急性淋巴细胞白血病伴前B细胞（CALLA + pre - B ALL）细胞的特征。T淋巴细胞白血病表达CD38、CD2、CD3、CD4和CD8标志物。在急性髓系白血病中可见CD36、CD13、CD14、CD15、CD11b和DR标志物的表达。表面标志物可确定白血病类型。白血病的免疫表型分析使我们能够做出精确诊断、确定其预后并选择合适的治疗方法。
