Weidmann Manfred, Zanotto Paolo M D A, Weber Friedemann, Spiegel Martin, Brodt Hans Rheinhard, Hufert Frank T
Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany.
J Clin Microbiol. 2004 Jun;42(6):2771-3. doi: 10.1128/JCM.42.6.2771-2773.2004.
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
与靶向聚合酶基因的扩增子相比,靶向严重急性呼吸综合征冠状病毒(SARS-CoV)核衣壳基因的Taqman扩增子对从感染细胞中提取的SARS-CoV靶RNA的敏感性高5个对数(10)倍,对从法兰克福首例病例的患者材料中提取的RNA的敏感性高2.79个对数(10)倍。