Ji Junfang, Zhao Liqun, Wang Xiuqin, Zhou Chuannong, Ding Fang, Su Lei, Zhang Chunlin, Mao Xuezheng, Wu Min, Liu Zhihua
National Lab of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, 100021 Beijing, P.R. China.
J Cancer Res Clin Oncol. 2004 Aug;130(8):480-6. doi: 10.1007/s00432-004-0555-x. Epub 2004 Jun 4.
To study the differential expression of the S100 gene family at the RNA level in human esophageal squamous cell carcinoma (ESCC), and to find the relationship of the S100 gene family with ESCC.
Firstly, the specific primers were designed for the different S100 genes with Software Primer 3, which required that both primer sequences of each S100 gene were from two different exons respectively. Then, the differential expression of 16 S100 genes was examined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in 62 cases of ESCC versus the corresponding normal esophageal mucosa. All RT-PCR products were analyzed by 1.5% agarose gel. With Fluor-S MultiImager and Multi-Analyst software, the electrophoresis images were evaluated with statistics analysis using SAS 8.1 software.
Eleven out of 16 S100 genes were significantly downregulated ( p<0.05) in ESCC versus the normal counterparts such as S100A1, S100A2, S100A4, S100A8, S100A9, S100A10, S100A11, S100A12, S100A14, S100B, and S100P genes. Only the S100A7 gene in the S100 family was markedly upregulated ( p<0.05). Moreover, the S100B gene was significantly correlated with histological differentiation of ESCC ( p=0.0247), and the deregulation of some S100 genes was closely correlated ( p<0.05), such as S100A10/S100A11, S100A2/S100A8, S100A2/S100A14, S100A8/S100A14, and S100A2/S100P etc.
The S100 gene family is closely associated with ESCC.
研究S100基因家族在人食管鳞状细胞癌（ESCC）中的RNA水平差异表达，并探寻S100基因家族与ESCC的关系。
首先，使用Primer 3软件为不同的S100基因设计特异性引物，要求每个S100基因的两条引物序列分别来自两个不同的外显子。然后，通过半定量逆转录聚合酶链反应（RT-PCR）检测62例ESCC及相应正常食管黏膜中16个S100基因的差异表达。所有RT-PCR产物用1.5%琼脂糖凝胶进行分析。使用Fluor-S MultiImager和Multi-Analyst软件，利用SAS 8.1软件对电泳图像进行统计学分析评估。
与正常对照相比，16个S100基因中有11个在ESCC中显著下调（p<0.05），如S100A1、S100A2、S100A4、S100A8、S100A9、S100A10、S100A11、S100A12、S100A14、S100B和S100P基因。S100家族中只有S100A7基因显著上调（p<0.05）。此外，S100B基因与ESCC的组织学分化显著相关（p=0.0247），一些S100基因的失调密切相关（p<0.05），如S100A10/S100A11、S100A2/S100A8、S100A2/S100A14、S100A8/S100A14和S100A2/S100P等。
S100基因家族与ESCC密切相关。
Zotero 插件