Albertyn J, Tajbhai K M, Bragg R R
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9300 South Africa.
Onderstepoort J Vet Res. 2004 Mar;71(1):29-34. doi: 10.4102/ojvr.v71i1.282.
Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes.
鹦鹉喙羽病（PBFD）是鹦鹉科鸟类的一种常见疾病，由鹦鹉喙羽病病毒（PBFDV）引起。在本研究中，使用聚合酶链反应作为诊断测试，调查了南非出现羽毛问题的环颈鹦鹉和虎皮鹦鹉中该疾病的发生情况。还研究了病毒分离株之间的遗传变异。结果表明，PBFDV可归因于南非一些环颈鹦鹉和虎皮鹦鹉出现羽毛问题的原因。分离株的遗传变异在物种和个体之间存在。还评估了一种用于诊断目的的在滤纸上采集血样的廉价且易于使用的方法。事实证明，这种方法对鸟类的压力较小，且不会抑制后续操作。
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