Faculty of Veterinary and Animal Sciences, Pir Mahr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Institute of Biochemistry and Biotechnology, Pir Mahr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Vet Res Commun. 2019 Nov;43(4):197-202. doi: 10.1007/s11259-019-09759-7. Epub 2019 Jul 11.
Avian polyomavirus (APV) infection, also called as budgerigar fledgling disease (BFD) causes various health problems in many psittacine species which may cause untimely death. The aims of this study were to investigate, for the first time, the detection, molecular characterization and phylogenetic analysis of avian polyomavirus (APV) in Pakistani psittacine birds. In an aviary a disease similar to APV was found and 90% of the nestlings died within a few weeks. Seven to ten-day-old parrot nestlings (n = 3) from the aviary were presented with feather abnormalities, plumage defect and were clinically depressed. Birds died at 11th, 14th and 16th day of age. Samples of hearts, livers, spleen, feathers and kidneys were collected from the dead birds. Samples were analyzed for the presence of APV DNA by using PCR. APV VP1 gene was partially sequenced, and phylogenetic analysis was performed. The APV strain was similar to those previously reported in other areas of the world. The results of this investigation indicate presence of a high frequency of APV infections in psittacine birds in Pakistan.
禽多瘤病毒（APV）感染，也称为虎皮鹦鹉幼鸟病（BFD），可导致许多凤头鹦鹉物种出现各种健康问题，并可能导致其过早死亡。本研究的目的是首次调查巴基斯坦凤头鹦鹉禽多瘤病毒（APV）的检测、分子特征和系统进化分析。在一个鸟舍中发现了一种类似于 APV 的疾病，巢中 90%的幼鸟在几周内死亡。来自鸟舍的 7 到 10 天大的鹦鹉幼鸟（n=3）出现羽毛异常、羽毛缺陷和临床抑郁等症状。鸟在第 11、14 和 16 天死亡。从死亡的鸟中采集心脏、肝脏、脾脏、羽毛和肾脏样本。使用 PCR 分析样本中是否存在 APV DNA。对 APV VP1 基因进行了部分测序，并进行了系统进化分析。该 APV 株与世界其他地区先前报道的株相似。该调查结果表明，APV 感染在巴基斯坦凤头鹦鹉中的发生率很高。
