Labruna Marcelo B, McBride Jere W, Bouyer Donal H, Camargo Luis Marcelo A, Camargo Erney P, Walker David H
Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootenia, Universidade de Sao Paulo, Brazil.
J Med Entomol. 2004 May;41(3):533-7. doi: 10.1603/0022-2585-41.3.533.
Two Amblyomma longirostre adult male ticks were collected from a Brazilian porcupine Coendou prehensilis L. in the state of Rondonia, Western Amazon, Brazil. The two ticks were pooled for DNA extraction and tested for the presence of rickettsial DNA by amplifying portions of the gltA, 17-kDa, ompA, and ompB rickettsial genes by polymerase chain reaction (PCR). Portions of the four genes were amplified from the sample and subsequently sequenced. These results indicated the presence of a Rickettsia strain infecting A. longirostre, which was designated as strain Aranha. Compared with homologous ompA rickettsial sequences, "Rickettsia amblyommii" ompA seemed to be the closest relative to Aranha (similarity values: 99.0-99.3%). Phylogenetic analyses of more conserved genes including 17-kDa and gtlA partial sequences indicated that this Rickettsia sp. is a spotted fever group rickettsia. The partial ompB sequence of strain Aranha was distinct from all homologous sequences available in GenBank. Although our ompA analysis suggested a very close molecular phylogenetic relationship of Aranha with "R. amblyommii," we cannot at this time determine if Aranha is a new strain of "R. amblyommii" or a new Rickettsia species in South America.
从巴西亚马逊西部朗多尼亚州的一只巴西帚尾豪猪（Coendou prehensilis L.）身上采集到两只长角花蜱（Amblyomma longirostre）成年雄蜱。将这两只蜱合并用于DNA提取，并通过聚合酶链反应（PCR）扩增立克次氏体基因的gltA、17-kDa、ompA和ompB部分片段，检测立克次氏体DNA的存在情况。从样本中扩增出了这四个基因的部分片段，随后进行了测序。这些结果表明存在一种感染长角花蜱的立克次氏体菌株，将其命名为阿兰哈菌株（Aranha）。与同源的立克次氏体ompA序列相比，“美洲花蜱立克次氏体”（Rickettsia amblyommii）的ompA似乎是与阿兰哈菌株亲缘关系最近的（相似性值：99.0 - 99.3%）。对包括17-kDa和gtlA部分序列在内的更保守基因进行系统发育分析表明，这种立克次氏体是斑点热群立克次氏体。阿兰哈菌株的ompB部分序列与GenBank中所有可用的同源序列都不同。尽管我们的ompA分析表明阿兰哈菌株与“美洲花蜱立克次氏体”有非常密切的分子系统发育关系，但目前我们无法确定阿兰哈菌株是“美洲花蜱立克次氏体”的一个新菌株还是南美洲的一个新立克次氏体物种。
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