Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, 04103 Leipzig, Germany.
Rio Negro Research and Transfer Center (CONICET-UNRN), National University of Rio Negro, 8500 Viedma, Argentina.
Parasitology. 2024 Jul;151(8):848-852. doi: 10.1017/S0031182024000933. Epub 2024 Oct 14.
The aim of this study was to examine the presence of tick-borne rickettsial bacteria in sensu stricto ticks collected from dogs in the Patagonian region of Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examined for the presence of s.s. ticks. Ninety ticks were collected and identified to species level. DNA was extracted and analysed by conventional PCR assays for the presence of tick-borne bacteria belonging to the genera , and . Thirty-three tick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceae bacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences were generated and belonging to , the causative agent of canine cyclic thrombocytopenia. Two tick samples were positive in specific PCR assays and were identified by phylogenetic analysis as , a member of the spotted fever group rickettsiae. The results of this study demonstrate the molecular detection of 2 rickettsial bacteria in s.s. in a region of Argentina where no data were available so far.
本研究旨在检测阿根廷巴塔哥尼亚地区从犬只身上采集的蜱中的蜱传立克次体细菌。从阿根廷里奥内格罗省瓦尔切塔的 14 只流浪犬身上检查了 s.s. 蜱的存在。采集了 90 只蜱并鉴定到种的水平。提取 DNA 并通过常规 PCR 检测分析了属于、和属的蜱传细菌。用不同的 PCR 检测方法对 33 个蜱虫池进行了检测，其中 3 个为立克次体科细菌阳性。在 3 个 Anaplasmataceae 阳性样本中，生成了 2 个部分 16S rDNA 序列，属于犬循环血小板减少症的病原体。2 个蜱虫样本在特定的 PCR 检测中呈阳性，并通过系统发育分析鉴定为斑点热群立克次体的成员。本研究结果证明了在阿根廷一个迄今尚无数据的地区，从 s.s. 中检测到了 2 种立克次体细菌。
