Tam Benny B, Siu Andrew W, Lam Andy F, Lee Elsa Y
Laboratory of Experimental Optometry, Department of Optometry and Radiography, The Hong Kong Polytechnic University, Kowloon, Hong Kong, China.
Clin Exp Optom. 2004 May;87(3):171-4. doi: 10.1111/j.1444-0938.2004.tb03170.x.
Pinoline is a pineal indoleamine naturally found in the retina. This study compared the effects of pinoline and vitamin E on the copper (I)-induced retinal lipid peroxidation (LPO).
Porcine retinal homogenates were mixed with 120 micro M copper (I) solution. The mixtures were co-incubated with various concentrations of pinoline or trolox (water-soluble vitamin E analogue) at 37 degrees Centigrade for 60 minutes. The amounts of malondialdehyde (MDA) and protein were assayed to quantify the LPO.
Copper (I) ions significantly increased the MDA concentration in the retinal homogenates (p < 0.0007). Both pinoline and trolox significantly suppressed MDA in a dose-dependent manner (p < 0.0001) and their effects were significantly different (p = 0.004). The concentrations that inhibited 50 per cent of LPO were 0.24 mM and 0.68 mM for pinoline and trolox, respectively.
Pinoline suppressed the LPO at a potency of 2.8 times compared with trolox. The results support an anti-oxidative role for pinoline in the retina. Further study is required to characterise the pharmacological potency of pinoline in vivo.
松果林碱是一种天然存在于视网膜中的松果体吲哚胺。本研究比较了松果林碱和维生素E对铜（I）诱导的视网膜脂质过氧化（LPO）的影响。
将猪视网膜匀浆与120微摩尔铜（I）溶液混合。将混合物在37摄氏度下与不同浓度的松果林碱或生育酚（水溶性维生素E类似物）共同孵育60分钟。测定丙二醛（MDA）和蛋白质的含量以量化LPO。
铜（I）离子显著增加了视网膜匀浆中的MDA浓度（p < 0.0007）。松果林碱和生育酚均以剂量依赖性方式显著抑制MDA（p < 0.0001），且它们的作用有显著差异（p = 0.004）。抑制50%LPO的松果林碱和生育酚的浓度分别为0.24毫摩尔和0.68毫摩尔。
与生育酚相比，松果林碱抑制LPO的效力高2.8倍。结果支持松果林碱在视网膜中的抗氧化作用。需要进一步研究以表征松果林碱在体内的药理效力。
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