使用含有和不含有重组牛粒细胞集落刺激因子的商业甲基纤维素培养基培养牛骨髓细胞。
Use of a commercial methylcellulose medium with and without recombinant bovine granulocyte colony-stimulating factor for culturing bovine bone marrow cells.
作者信息
Keller Sonya L, Wood R Darren, Jefferson Barbara J, Jacobs Robert M
机构信息
Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1.
出版信息
Can J Vet Res. 2004 Apr;68(2):157-9.
A commercial methylcellulose culture medium, with and without the addition of recombinant bovine granulocyte colony-stimulating factor (rbG-CSF), was utilized for culturing bovine bone marrow cells in a colony-forming unit assay. Bone marrow mononuclear cells were isolated and cultured in a commercial methylcellulose-based medium containing several recombinant human cytokines. Cultures were prepared with and without 100 ng/mL of rbG-CSF. The size and mean number of colonies per plate from culture days 3 to 9 were compared. We concluded that bovine bone marrow colony growth was supported by this culture medium. The addition of rbG-CSF yielded larger and more numerous colonies. There were significantly more colonies on day 3 (P < 0.001), day 4 (P < 0.001), and day 5 (P = 0.03) with rbG-CSF. Both culture media had the highest colony counts on day 5.
在集落形成单位测定中，使用一种商业甲基纤维素培养基，分别添加和不添加重组牛粒细胞集落刺激因子（rbG-CSF）来培养牛骨髓细胞。分离骨髓单个核细胞，并在含有几种重组人细胞因子的基于商业甲基纤维素的培养基中培养。分别制备添加和不添加100 ng/mL rbG-CSF的培养物。比较培养第3天至第9天每平板上集落的大小和平均数量。我们得出结论，这种培养基支持牛骨髓集落生长。添加rbG-CSF可产生更大、更多的集落。添加rbG-CSF时，在第3天（P < 0.001）、第4天（P < 0.001）和第5天（P = 0.03）集落明显更多。两种培养基在第5天的集落计数最高。
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