McNiece I K, Langley K E, Zsebo K M
Amgen Inc., Thousand Oaks, California 91320-1789.
Exp Hematol. 1991 Mar;19(3):226-31.
The cDNA for human stem cell factor (hSCF) has been cloned and expressed in mammalian and bacterial hosts and recombinant protein purified. We have examined the stimulatory effect of recombinant human SCF (rhSCF) on human bone marrow cells alone and in combination with recombinant human colony stimulating factors (CSFs) and erythropoietin (rhEpo). RhSCF alone resulted in no significant colony formation, however, in the presence of rhGM-CSF, rhG-CSF or rhIL-3, rhSCF stimulated a synergistic increase in colony numbers. In addition, increased colony size was stimulated by all combinations. The morphology of cells in the colonies obtained with the CSFs plus rhSCF was identical to the morphology obtained with rhGM-CSF, rhG-CSF or rhIL-3 alone. RhEpo also synergised with rhSCF to stimulate the formation of large compact hemoglobinized colonies which stained positive for spectrin and transferrin receptor and had a morphological appearance consistent with normoblasts. RhSCF stimulation of low density non-adherent, antibody depleted, CD34+ cells suggests that rhSCF directly stimulates progenitor cells capable of myeloid and erythroid differentiation.
人干细胞因子（hSCF）的互补DNA（cDNA）已被克隆，并在哺乳动物和细菌宿主中表达，且重组蛋白已被纯化。我们研究了重组人SCF（rhSCF）单独以及与重组人集落刺激因子（CSF）和促红细胞生成素（rhEpo）联合使用时对人骨髓细胞的刺激作用。单独使用rhSCF不会导致显著的集落形成，然而，在存在rhGM-CSF、rhG-CSF或rhIL-3的情况下，rhSCF会刺激集落数量协同增加。此外，所有组合都会刺激集落大小增加。用CSF加rhSCF获得的集落中的细胞形态与单独用rhGM-CSF、rhG-CSF或rhIL-3获得的形态相同。RhEpo也与rhSCF协同作用，刺激形成大而紧密的血红蛋白化集落，这些集落对血影蛋白和转铁蛋白受体染色呈阳性，并且形态外观与正成红细胞一致。rhSCF对低密度非贴壁、抗体清除的CD34+细胞的刺激表明，rhSCF直接刺激能够进行髓系和红系分化的祖细胞。
