Young K M, Moriello K A, Peickert H
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
Am J Vet Res. 1997 Apr;58(4):348-53.
To identify eosinophil progenitor cells in feline bone marrow, establishing an assay method to use in studies of eosinophilopoiesis and eosinophilopoietic factors in cats.
Healthy, laboratory animal source cats.
Sources of colony-stimulating activity were prepared by conditioning media with bone marrow, spleen, and blood mononuclear cells from cats infected with Toxocara canis. Bone marrow cells were aspirated and cultured to develop the eosinophil progenitor cell assay and to test cells from 9 healthy cats in the assay.
Optimal conditions for identifying colony-forming units-eosinophil and cluster-forming units-eosinophil were as follows. Bone marrow mononuclear cells (10(5)) were plated in 1 ml of supplemented medium, fetal bovine serum, and agar. The source of eosinophil growth factor(s) was bone marrow-conditioned medium made in the presence of 2.5 micrograms of concanavalin A/ml; other conditioned media also supported eosinophil colony growth. Dishes were incubated for 7 days at 37 C and 7% CO2. The colony-forming units-eosinophil formed aggregates of > 50 Luxol fast blue-positive cells and had dispersed morphology; the cluster-forming units-eosinophil formed aggregates of < 50 cells.
Similar to other species, cats have separate and distinct eosinophil progenitor cells. The eosinophil progenitor assay may be used to characterize altered kinetics of eosinophilopoiesis, to assess eosinophil growth factors, and to evaluate therapeutic regimens that might be useful in the management of excess eosinophil production.
鉴定猫骨髓中的嗜酸性粒细胞祖细胞，建立一种用于猫嗜酸性粒细胞生成及嗜酸性粒细胞生成因子研究的检测方法。
健康的实验动物来源猫。
通过用感染犬弓首蛔虫的猫的骨髓、脾脏和血液单核细胞对培养基进行预处理来制备集落刺激活性来源。抽取骨髓细胞并进行培养，以建立嗜酸性粒细胞祖细胞检测方法，并对9只健康猫的细胞进行该检测。
鉴定嗜酸性粒细胞集落形成单位和嗜酸性粒细胞簇形成单位的最佳条件如下。将骨髓单个核细胞（10⁵）接种于1 ml添加了胎牛血清和琼脂的培养基中。嗜酸性粒细胞生长因子的来源是在含有2.5 μg/ml伴刀豆球蛋白A的情况下制备的骨髓条件培养基；其他条件培养基也支持嗜酸性粒细胞集落生长。培养皿在37℃、7%二氧化碳条件下孵育7天。嗜酸性粒细胞集落形成单位形成了大于50个Luxol固蓝阳性细胞的聚集体，形态分散；嗜酸性粒细胞簇形成单位形成了小于50个细胞的聚集体。
与其他物种相似，猫具有独立且不同的嗜酸性粒细胞祖细胞。嗜酸性粒细胞祖细胞检测可用于表征嗜酸性粒细胞生成动力学的改变、评估嗜酸性粒细胞生长因子以及评估可能对控制嗜酸性粒细胞过度生成有用的治疗方案。
