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[婴儿早期定植口腔微生物群的分析]

[Analysis of oral microflora early colonized in infants].

作者信息

Zou Jing, Zhou Xue-dong, Li Shao-min

机构信息

Dept. of Pediatric Dentistry, West China College of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2004 Apr;22(2):126-8.

Abstract

OBJECTIVE

To observe the dynamic changes of oral microflora early colonized in infants.

METHODS

The oral swab samples for the study were taken in 1 day, 1, 3, 6, 9, 12 months after birth from 12 healthy neonates. By choosing suitable diluted concentration, the samples were incubated aerobically, facultative anaerobically and anaerobically. The strains were identified by observing colony characteristics, Gram staining and biochemical tests.

RESULTS

S. salivarius was the most frequent microflora, followed by S. mitis, S. sanguis, S. gordonii and S. mutans occurred in oral cavity after tooth eruption. Veillonella spp. can be detected in oral cavity of 1-month-old babies, A. odontolyticus was isolated from 8.3% infants of more than 3 months old. L. acidophilus maintained the lower prevalence in oral cavity of babies. Leptotrichia buccalis and Capnocytophaga spp. occurred in oral cavity of some dentate infants.

CONCLUSION

S. solivarius and S. mitis are predominant species in oral cavity of the infants, Veillonella spp. is the first and the most anaerobic species appeared in oral cavity of healthy babies. A. odontolyticus is the first actinomyces detected in oral cavity. With the increasing months, kind and amount of microflora increase dramatically.

摘要

目的

观察早期定植于婴儿口腔的微生物群落动态变化。

方法

选取12例健康新生儿,分别于出生后1天、1、3、6、9、12个月采集口腔拭子样本。选择合适的稀释浓度后,将样本分别进行需氧培养、兼性厌氧培养和厌氧培养。通过观察菌落特征、革兰氏染色及生化试验对菌株进行鉴定。

结果

唾液链球菌是最常见的微生物,其次是缓症链球菌、血链球菌、戈登链球菌,变形链球菌在牙齿萌出后出现在口腔中。韦荣氏菌属可在1月龄婴儿口腔中检测到,溶齿放线菌从3个月以上婴儿中分离出的比例为8.3%。嗜酸乳杆菌在婴儿口腔中的患病率维持在较低水平。颊纤毛菌和二氧化碳嗜纤维菌出现在部分有牙婴儿的口腔中。

结论

唾液链球菌和缓症链球菌是婴儿口腔中的优势菌,韦荣氏菌属是健康婴儿口腔中最早出现且最主要的厌氧菌。溶齿放线菌是在口腔中检测到的第一种放线菌。随着月龄增加,微生物的种类和数量显著增加。

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