Almståhl A, Wikström M, Fagerberg-Mohlin B
Department of Oral Microbiology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Oral Dis. 2008 Sep;14(6):541-9. doi: 10.1111/j.1601-0825.2007.01416.x. Epub 2008 Jan 17.
To analyse the microbial flora in specific oral sites in 13 dentate subjects, 6-8 months after completed radiation therapy (RT group) and in 13 matched controls.
The microflora on the tongue, buccal mucosa, vestibulum, supragingival plaque and subgingival region was analysed using duplicate sampling and cultivation technique. A clinical examination was also performed.
Candida albicans was found in one or more sites in 54% of the RT subjects and in 15% of the controls. In three RT subjects, C. albicans was found at all four sites analysed. An unexpected finding was that enterococci were found in all RT subjects and in high number in 38%. None of the controls harboured enterococci. In supragingival plaque, Lactobacillus spp. were detected in 92% of the RT subjects and the number and proportion of Lactobacillus spp. were extremely high compared with the controls. Mutans streptococci were detected in high numbers in 31% of the RT subjects, while they were not detected in 23%.
The microbial results explain why some RT subjects have an increased susceptibility to oral diseases and stress that site-specific microbial analysis is an important diagnostic tool when planning oral health preventive care for RT subjects.
分析13名有牙受试者在完成放射治疗6 - 8个月后的特定口腔部位的微生物菌群（放疗组），并与13名匹配的对照组进行比较。
采用重复采样和培养技术分析舌、颊黏膜、前庭、龈上菌斑和龈下区域的微生物菌群。同时进行临床检查。
54%的放疗组受试者在一个或多个部位发现白色念珠菌，而对照组这一比例为15%。在三名放疗组受试者中，在所有四个分析部位均发现白色念珠菌。一个意外发现是，所有放疗组受试者均发现肠球菌，38%的受试者肠球菌数量较多。对照组未发现肠球菌。在龈上菌斑中，92%的放疗组受试者检测到乳酸杆菌属，与对照组相比，乳酸杆菌属的数量和比例极高。31%的放疗组受试者检测到变形链球菌数量较多，而23%的受试者未检测到。
微生物检测结果解释了为什么一些放疗组受试者对口腔疾病的易感性增加，并强调在为放疗组受试者规划口腔健康预防护理时，特定部位的微生物分析是一种重要的诊断工具。
