Könönen E, Asikainen S, Saarela M, Karjalainen J, Jousimies-Somer H
Institute of Dentistry, Department of Periodontology, University of Helsinki, Finland.
Oral Microbiol Immunol. 1994 Jun;9(3):136-41. doi: 10.1111/j.1399-302x.1994.tb00049.x.
Eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. The composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. The bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). During the primary period, 2 samples were collected from each child: a sample with dental floss from gingival margin of 2 teeth and stimulated saliva pooled with a mucosal swab sample. Both samples were cultured aerobically and anaerobically using nonselective and selective media. Prevotella melaninogenica, nonpigmented Prevotella spp., Fusobacterium nucleatum group and Capnocytophaga spp. were found in all children at the older age, whereas they occurred in edentulous mouth in 76%, 62%, 67% and 19%, respectively. The occurrence of Prevotella loescheii increased from 14% to 90%, Prevotella intermedia from 10% to 67%, Leptotrichia spp. from 24 to 71%, Campylobacter (Wolinella) spp. from 5 to 43% and Eikenella corrodens from 5 to 57%. Only the occurrence of Bacteroides gracilis and Veillonella spp. remained at about the same level. Species not isolated from the edentulous mouth, such as Prevotella denticola, Fusobacterium spp. other than the F. nucleatum group and Selenomonas spp. were now detected in 71%, 71% and 43% of the children. The stability of the colonizing P. melaninogenica strain(s) in the oral cavity was determined by using ribotyping; 1-2 isolates per child from the edentulous infant period of 9 children and 3-15 isolates per child from their primary dentition period were analyzed.(ABSTRACT TRUNCATED AT 250 WORDS)
乳牙萌出通过为细菌定植提供适宜的生态位，对口腔环境有很大影响。本研究调查了21名乳牙期幼儿（平均年龄32个月）口腔革兰氏阴性厌氧微生物区系的组成。将样本的细菌检测结果与这些儿童在无牙婴儿期（平均年龄3个月）采集的样本进行比较。在乳牙期，从每个儿童采集2份样本：一份用牙线从2颗牙齿的牙龈边缘采集，另一份用黏膜拭子采集刺激唾液样本。两份样本均使用非选择性和选择性培养基进行需氧和厌氧培养。在所有年龄较大的儿童中均发现了产黑色素普雷沃菌、无色素普雷沃菌属、具核梭杆菌群和二氧化碳嗜纤维菌属，而在无牙口腔中，它们的出现率分别为76%、62%、67%和19%。洛氏普雷沃菌的出现率从14%增至90%，中间普雷沃菌从10%增至67%，纤毛菌属从24%增至71%，弯曲菌（沃氏菌属）从5%增至43%，腐蚀艾肯菌从5%增至57%。只有纤细拟杆菌和韦荣球菌属的出现率保持在大致相同水平。在71%、71%和43%的儿童中检测到了在无牙口腔中未分离出的菌种，如齿龈普雷沃菌、具核梭杆菌群以外的梭杆菌属和栖瘤胃普雷沃菌属。通过核糖体分型确定了口腔中产黑色素普雷沃菌菌株的定植稳定性；分析了9名儿童无牙婴儿期每个儿童1 - 2株分离菌以及乳牙期每个儿童3 - 15株分离菌。（摘要截断于250字）
