[多参数流式细胞术对外周血白血病细胞的免疫表型分析]

[Immunophenotyping of peripheral blood leukemic cells by multi-parameter cytometry].

作者信息

Zhang Ti, Zhao Ling-ling, Zhang Chun-qing, Xu Cong-gao

机构信息

Hematology Research Laboratory, Qilu Hospital, Shandong University, Jinan 250012, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Mar;20(2):225-7.

PMID:15191732

Abstract

AIM

To explore the clinical application and significance of CD45/SCC gating strategy of multi-parameter cytometry and tricolor fluorescence-labeled monoclonal antibodies in immunophenotyping of peripheral blood leukocytes in leukemia.

METHODS

Peripheral blood leukocytes were stained with CD45 combined with a panel of monoclonal antibodies to different lineage markers. CD45/SSC dot plot was formed and leukemic cell colony, once displayed, was gated for immunophenotyping.

RESULTS

144 patients with acute leukemia and 51 with chronic leukemia were enrolled. Distinguishable leukemic cell colony was found in 137 out of 144 specimens. 66 patients were immunologically classified as ALL and 67 as AML. 4 patients were unable to be classified immunophenotypically. 27 AL patients coexpressed markers of another cell lineage (20%). Immunophenotypic features of different types of leukemia were described. It is a common phenomenon that AL of one lineage coexpressed markers of another lineage.

CONCLUSION

An accurate immunophenotyping of leukemia can be realised when leukemic cell colony is displayed and analyzed by CD45/SCC pattern and gating strategy, which is important for clinical treatment and prognosis judgment.

摘要

目的

探讨多参数细胞术的CD45/SCC门控策略及三色荧光标记单克隆抗体在白血病外周血白细胞免疫表型分析中的临床应用及意义。

方法

外周血白细胞用CD45与一组针对不同谱系标志物的单克隆抗体进行染色。形成CD45/SSC点图，一旦显示出白血病细胞集落，就对其进行门控以进行免疫表型分析。

结果

纳入144例急性白血病患者和51例慢性白血病患者。144份标本中有137份发现了可区分的白血病细胞集落。66例患者免疫分类为ALL，67例为AML。4例患者无法进行免疫表型分类。27例急性白血病患者共表达另一种细胞谱系的标志物（20%）。描述了不同类型白血病的免疫表型特征。一个谱系的急性白血病共表达另一个谱系的标志物是一种常见现象。