Miyazaki T, Nishikawa K, Noma Y, Tsukaguchi N, Nakagawa K, Kobayashi Y, Kohsaka T
Otsuka Tokyo Assay Laboratories, Japan.
Rinsho Ketsueki. 1996 Mar;37(3):214-20.
Gating of leukemic cells for immunophenotyping by flow cytometry is currently set on two dimensional display of forward scatter (FSC) and side scatter (SSC). However, this gating method can not descriminate leukemic cells from contaminated normal cells (especially normal lymphocytes). Thus, in this paper, we used CD45 monoclonal antibody to detect leukemic cells (CD45dim cells) in conjunction with SSC parameter. This CD45-SSC gating revealed that this method is very useful in AML and ALL, especially leukemia with low percentage blasts. We recommend that this new gating method should be employed for more accurate immunophenotyping of acute leukemia.
目前，通过流式细胞术进行免疫表型分析时，白血病细胞的设门是基于前向散射（FSC）和侧向散射（SSC）的二维显示。然而，这种设门方法无法将白血病细胞与污染的正常细胞（尤其是正常淋巴细胞）区分开来。因此，在本文中，我们使用CD45单克隆抗体结合SSC参数来检测白血病细胞（CD45dim细胞）。这种CD45-SSC设门显示该方法在急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）中非常有用，尤其是原始细胞比例低的白血病。我们建议采用这种新的设门方法来更准确地进行急性白血病的免疫表型分析。
