[排卵后老化对小鼠卵母细胞姐妹染色单体平衡前期分离的影响]

[Influence of postovulatory ageing on balanced predivision of sister chromatid in mouse oocytes].

作者信息

Chen Wen, Liu Qun, Li Zhou, Wang Xin-rong, Zhu Gui-jin

机构信息

Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030 PR China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Jun;23(3):256-9.

PMID:16767658

Abstract

OBJECTIVE

To study the impact of postovulatory ageing to balanced predivision of oocyte sister chromatid.

METHODS

The mouse oocytes were cultured 0-72 h. Then chromosome 16 was detected by fluorescence in situ hybridization (FISH). The oocyte spindle and chromosome configuration were examined by immunocytochemistry.

RESULTS

For freshly ovulated mouse oocyte, the balanced predivision of sister chromatid occurred only at 7%. However, for oocytes cultured for 24 h, 48 h and 72 h in vitro, the balanced predivision of sister chromatid occurred up to at 32%, 51% or 62% respectively (P< 0.01). The abnormal cell spindle and chromosome configuration occurred at 9% of freshly ovulated oocytes, but it increased to 63%, 83% and 98% when the oocytes were cultured in vitro for 24 h, 48 h or 72 h respectively (P< 0.01).

CONCLUSION

The occurrence of balanced predivision of oocyte sister chromatid may result during postovulatory ageing, and may be related to change of oocyte spindle and chromosome configuration.

摘要

目的

研究排卵后老化对卵母细胞姐妹染色单体平衡前期分离的影响。

方法

将小鼠卵母细胞培养0 - 72小时。然后通过荧光原位杂交（FISH）检测16号染色体。通过免疫细胞化学检查卵母细胞纺锤体和染色体构型。

结果

对于刚排卵的小鼠卵母细胞，姐妹染色单体的平衡前期分离仅发生在7%。然而，对于在体外培养24小时、48小时和72小时的卵母细胞，姐妹染色单体的平衡前期分离分别高达32%、51%或62%（P < 0.01）。9%的刚排卵卵母细胞出现异常细胞纺锤体和染色体构型，但当卵母细胞在体外分别培养24小时、48小时或72小时时，这一比例分别增加到63%、83%和98%（P < 0.01）。