Smits Simone M, Terwisscha van Scheltinga Afke F, van der Linden Annemarie J A, Burbach J Peter H, Smidt Marten P
Rudolf Magnus Institute of Neuroscience, Department of Pharmacology and Anatomy, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, The Netherlands.
Brain Res Mol Brain Res. 2004 Jun 18;125(1-2):22-8. doi: 10.1016/j.molbrainres.2004.03.001.
We have used in situ hybridization to determine the distribution of pre-pro-neurotensin/neuromedin N (NT/N) mRNA in the brain of mice and rats. In rats and mice, expression was observed in the lateral septal nucleus, nucleus accumbens, medial preoptic area, bed nucleus of the stria terminalis, lateral hypothalamus, central amygdaloid nucleus and the subicilum. However, several differences in the NT/N mRNA distribution were observed between rats and mice in other brain areas. In mice, NT/N expression was detected in the subthalamic nucleus and geniculate nucleus, whereas expression was not observed in these brain areas in rats. Surprisingly, expression was not observed in mouse mesencephalic dopaminergic (mesDA) neurons and the CA1 area of the hippocampus, areas known to contain NT/N mRNA in the rat brain. Taken together, these results show that although the brain NT/N mRNA distribution largely overlaps in mice and rats, species differences exist in specific brain areas in rodents. Moreover, these data indicate that the distribution in mice resembles most that of primates than rats.
我们已使用原位杂交技术来确定前神经降压素/神经介素N（NT/N）前体mRNA在小鼠和大鼠大脑中的分布。在大鼠和小鼠中，可在外侧隔核、伏隔核、内侧视前区、终纹床核、下丘脑外侧区、中央杏仁核和海马下脚观察到表达。然而，在大鼠和小鼠的其他脑区中，观察到NT/N mRNA分布存在一些差异。在小鼠中，可在丘脑底核和膝状核中检测到NT/N表达，而在大鼠的这些脑区中未观察到表达。令人惊讶的是，在小鼠中脑多巴胺能（mesDA）神经元和海马CA1区未观察到表达，而在大鼠脑中已知这些区域含有NT/N mRNA。综上所述，这些结果表明，尽管小鼠和大鼠大脑中NT/N mRNA的分布大部分重叠，但在啮齿动物的特定脑区存在物种差异。此外，这些数据表明，与大鼠相比，小鼠中的分布与灵长类动物的分布最为相似。
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