Johnson N, Letshwenyo M, Baipoledi E K, Thobokwe G, Fooks A R
Rabies Research and Diagnostic Group, WHO Collaborating Centre for the Characterisation of Rabies and Rabies-Related Viruses, Veterinary Laboratories Agency, Weybridge, Woodham Lane, Addlestone KT15 3NB, UK.
Vet Microbiol. 2004 Jun 10;101(1):31-8. doi: 10.1016/j.vetmic.2004.03.007.
A panel of rabies virus isolates (RABV) endemic within Botswana between 1988 and 1992 have been typed by anti-nucleocapsid monoclonal antibodies (MAb) into two dominant groups. The first associated with the domestic dog (Canis familiaris) and the second associated with a range of wildlife species. Using nucleoprotein coding sequence data, we have applied molecular phylogenetic techniques to the same panel of 35 well-characterised rabies virus isolates from throughout Botswana in an attempt to compare both techniques and to further investigate the virus/host species relationships within this African country. The results confirm that there are indeed two major groups and that these are related primarily to biotype. The wildlife-associated biotype appeared more phylogenetically diverse and was more commonly isolated in the southeast of the country, with the canine-associated group dominating the north of the country. In addition, molecular phylogeny identified further groupings within both biotypes and a small number of isolates, which were not classified by MAb typing, could be assigned to a group. During the study period (4 years) there appeared to be little sequence variation within groups suggesting that distinct lineages persisted throughout the study and that there appears to be little evolutionary pressure on the nucleoprotein coding region of the viral genome.
1988年至1992年间在博茨瓦纳流行的一组狂犬病病毒分离株（RABV），已通过抗核衣壳单克隆抗体（MAb）分为两个主要组。第一组与家犬（犬属）有关，第二组与一系列野生动物物种有关。利用核蛋白编码序列数据，我们对来自博茨瓦纳各地的35株特征明确的狂犬病病毒分离株进行了分子系统发育技术分析，试图比较这两种技术，并进一步研究这个非洲国家内病毒/宿主物种的关系。结果证实确实存在两个主要组，且这些组主要与生物型有关。与野生动物相关的生物型在系统发育上似乎更多样化，且在该国东南部更常见，而与犬类相关的组在该国北部占主导地位。此外，分子系统发育在两种生物型中都识别出了进一步的分组，并且少数未通过MAb分型分类的分离株也可以归为一个组。在研究期间（4年），组内似乎几乎没有序列变异，这表明在整个研究过程中不同的谱系持续存在，并且病毒基因组的核蛋白编码区域似乎几乎没有进化压力。
