在肌萎缩侧索硬化症患者的脑脊液中检测不到白细胞介素-12和干扰素-γ。

Interleukin-12 and interferon-gamma are not detectable in the cerebrospinal fluid of patients with amyotrophic lateral sclerosis.

作者信息

Ford Lara, Rowe Dominic

机构信息

Northern Clinical School, Faculty of Medicine, University of Sydney, Australia.

出版信息

Amyotroph Lateral Scler Other Motor Neuron Disord. 2004 Jun;5(2):118-20. doi: 10.1080/14660820410019125.

DOI:10.1080/14660820410019125

PMID:15204013

Abstract

The cause of sporadic amyotrophic lateral sclerosis (SALS) is unknown. We investigated the immune-mediated inflammatory hypothesis of SALS by assaying interleukin-12 (IL-12), interleukin-6 (IL-6) and interferon-gamma (IFN-gamma) in the cerebrospinal fluid (CSF) of patients with SALS. These cytokines were measured in the CSF from patients with SALS (n=11), patients with immune-mediated inflammatory central nervous system or nerve root disorders (n=12), and patients with other neurological diseases (n=15) by high sensitivity sandwich enzyme linked immunosorbent assay (ELISA). All samples were below the assay detection limits of 0.5 pg/ml for IL-12 and 8 pg/ml for IFN-gamma. There was no difference between the groups in the mean concentration of IL-6. There is no evidence in cerebrospinal fluid for induction of a T(H)1 immune response in SALS.

摘要

散发性肌萎缩侧索硬化症（SALS）的病因尚不清楚。我们通过检测SALS患者脑脊液（CSF）中的白细胞介素-12（IL-12）、白细胞介素-6（IL-6）和干扰素-γ（IFN-γ），研究了SALS的免疫介导炎症假说。采用高灵敏度夹心酶联免疫吸附测定（ELISA）法，对11例SALS患者、12例免疫介导炎症性中枢神经系统或神经根疾病患者以及15例其他神经系统疾病患者的脑脊液中的这些细胞因子进行了检测。所有样本中IL-12的浓度均低于0.5 pg/ml的检测限，IFN-γ的浓度均低于8 pg/ml的检测限。各组间IL-6的平均浓度无差异。脑脊液中没有证据表明SALS患者存在T（H）1免疫反应的诱导。