Moustafa M, Simpson C, Glover M, Dawson R A, Tesfaye S, Creagh F M, Haddow D, Short R, Heller S, MacNeil S
Section of Human Metabolism, Division of Clinical Sciences (North), Northern General Hospital, Sheffield S5 7AU, UK.
Diabet Med. 2004 Jul;21(7):786-9. doi: 10.1111/j.1464-5491.2004.01166.x.
To evaluate the use of a new cell-tailored carrier surface (TranCell) for delivery of autologous keratinocytes to promote wound healing in patients with chronic neuropathic foot ulcers.
TranCell is a sterile medical grade polymer coated with a plasma-polymerized functional surface containing 20% carboxylic acid which enables keratinocytes to attach and proliferate. Six diabetic patients with neuropathic ulcers resistant to conventional therapy were treated with weekly applications of autologous keratinocytes delivered on TranCell. A split-thickness skin biopsy was taken from each patient followed by isolation, expansion and freezing down of keratinocytes. Keratinocytes were thawed and seeded on TranCell 48 h prior to application. This procedure was repeated weekly in addition to conventional therapy until wound healing was achieved.
Complete healing was achieved in six out of nine ulcers in six patients, a reduction in ulcer size was achieved in one ulcer and no response was seen in one ulcer. Treatment was discontinued in one patient due to development of Methicillin-Resistant Staphylococcus aureus (MRSA) after only three applications of TranCell. Wound healing took 6-17 applications over 6-20 weeks. There were no recurrences in the healed ulcers after a follow-up of 6 months.
TranCell delivery of autologous cells is a promising treatment for chronic diabetic foot ulcers with no side-effects and no recurrence in the healed ulcers.
评估一种新型细胞定制载体表面（TranCell）用于递送自体角质形成细胞以促进慢性神经性足部溃疡患者伤口愈合的效果。
TranCell是一种无菌医用级聚合物，表面涂有含20%羧酸的等离子体聚合功能表面，可使角质形成细胞附着并增殖。6例对传统治疗耐药的神经性溃疡糖尿病患者，每周接受在TranCell上递送的自体角质形成细胞治疗。从每位患者身上取分层皮肤活检样本，随后分离、扩增并冻存角质形成细胞。在应用前48小时将角质形成细胞解冻并接种在TranCell上。除传统治疗外，每周重复此过程，直至伤口愈合。
6例患者的9处溃疡中有6处完全愈合，1处溃疡面积缩小，1处溃疡无反应。1例患者仅在应用3次TranCell后因耐甲氧西林金黄色葡萄球菌（MRSA）感染而停止治疗。伤口愈合在6至20周内进行了6至17次应用。随访6个月后，愈合的溃疡无复发。
TranCell递送自体细胞是治疗慢性糖尿病足溃疡的一种有前景的方法，无副作用，愈合的溃疡无复发。
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