Kroto Research Institute, Department of Engineering Materials, University of Sheffield, Broad Lane, Sheffield, United Kingdom.
Tissue Eng Part C Methods. 2012 Feb;18(2):143-55. doi: 10.1089/ten.TEC.2011.0037. Epub 2011 Nov 10.
Skin has a remarkable capacity for regeneration, but age- and diabetes-related vascular problems lead to chronic non-healing wounds for many thousands of U.K. patients. There is a need for new therapeutic approaches to treat these resistant wounds. Donor mesenchymal stem/stromal cells (MSCs) have been shown to assist cutaneous wound healing by accelerating re-epithelialization. The aim of this work was to devise a low risk and convenient delivery method for transferring these cells to wound beds. Plasma polymerization was used to functionalize the surface of medical-grade silicone with acrylic acid. Cells attached well to these carriers, and culture for up to 3 days on the carriers did not significantly affect their phenotype or ability to support vascular tubule formation. These carriers were then used to transfer MSCs onto human dermis. Cell transfer was confirmed using an MTT assay to assess viable cell numbers and enhanced green fluorescent protein-labeled MSCs to demonstrate that the cells post-transfer attached to the dermis. We conclude that this synthetic carrier membrane is a promising approach for delivery of therapeutic MSCs and opens the way for future studies to evaluate its impact on repairing difficult skin wounds.
皮肤具有非凡的再生能力，但由于年龄和糖尿病相关的血管问题，成千上万的英国患者会出现慢性难愈性伤口。因此，需要新的治疗方法来治疗这些耐药性伤口。供体间充质干细胞（MSCs）已被证明可通过加速再上皮化来促进皮肤伤口愈合。本研究旨在设计一种低风险且方便的方法，将这些细胞转移到伤口床。通过等离子体聚合将医用级硅酮表面功能化接枝丙烯酸。细胞很好地附着在这些载体上，在载体上培养长达 3 天不会显著影响其表型或支持血管小管形成的能力。然后，这些载体被用于将 MSCs 转移到人体真皮上。通过 MTT 测定法证实细胞转移，以评估活细胞数量，并使用增强型绿色荧光蛋白标记的 MSCs 证明转移后细胞附着在真皮上。我们得出结论，这种合成载体膜是一种有前途的治疗性 MSCs 传递方法，为未来的研究评估其对修复困难皮肤伤口的影响开辟了道路。
