影响青鳉视网膜发育的突变

Mutations affecting retina development in Medaka.

作者信息

Loosli Felix, Del Bene Filippo, Quiring Rebecca, Rembold Martina, Martinez-Morales Juan-Ramon, Carl Matthias, Grabher Clemens, Iquel Caroline, Krone Annette, Wittbrodt Beate, Winkler Sylke, Sasado Takao, Morinaga Chikako, Suwa Hiroshi, Niwa Katsutoshi, Henrich Thorsten, Deguchi Tomonori, Hirose Yukihiro, Iwanami Norimasa, Kunimatsu Sanae, Osakada Masakazu, Watanabe Tomomi, Yasuoka Akihito, Yoda Hiroki, Winkler Christoph, Elmasri Harun, Kondoh Hisato, Furutani-Seiki Makoto, Wittbrodt Joachim

机构信息

European Molecular Biology Laboratory, Developmental Biology Programme, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

出版信息

Mech Dev. 2004 Jul;121(7-8):703-14. doi: 10.1016/j.mod.2004.03.004.

DOI:10.1016/j.mod.2004.03.004

PMID:15210178

Abstract

In a large scale mutagenesis screen of Medaka we identified 60 recessive zygotic mutations that affect retina development. Based on the onset and type of phenotypic abnormalities, the mutants were grouped into five categories: the first includes 11 mutants that are affected in neural plate and optic vesicle formation. The second group comprises 15 mutants that are impaired in optic vesicle growth. The third group includes 18 mutants that are affected in optic cup development. The fourth group contains 13 mutants with defects in retinal differentiation. 12 of these have smaller eyes, whereas one mutation results in enlarged eyes. The fifth group consists of three mutants with defects in retinal pigmentation. The collection of mutants will be used to address the molecular genetic mechanisms underlying vertebrate eye formation.

摘要

在对青鳉进行的大规模诱变筛选中，我们鉴定出60个影响视网膜发育的隐性合子突变。根据表型异常的发生时间和类型，这些突变体被分为五类：第一类包括11个在神经板和视泡形成过程中受到影响的突变体。第二类包含15个视泡生长受损的突变体。第三类包括18个在视杯发育过程中受到影响的突变体。第四类有13个视网膜分化存在缺陷的突变体。其中12个眼睛较小，而一个突变导致眼睛增大。第五类由三个视网膜色素沉着存在缺陷的突变体组成。这些突变体的集合将用于研究脊椎动物眼睛形成的分子遗传机制。