核酸外切酶A5的核糖核酸酶和脱氧核糖核酸酶活性。

Ribonuclease and deoxyribonuclease activity of exonuclease A5.

作者信息

L'vova T N, Tatarskaya R I

出版信息

Mol Biol (Mosk). 1976 Jul-Aug;10(4):652-7.

Abstract

A comparative study was made of the specificity and some properties of the RNase and DNase activities of exonuclease A5. The results obtained indicate that RNA and denatured DNA are hydrolyzed in the same active center of the enzyme and, consequently, exonuclease A5 is an enzyme which is not specific for the sugar of nucleic acids. The data reported are important when exonuclease A5 is used as a specific reagent in investigations of nucleic acids.

摘要

对外切核酸酶A5的核糖核酸酶和脱氧核糖核酸酶活性的特异性及某些特性进行了比较研究。所得结果表明，RNA和变性DNA在该酶的同一个活性中心被水解，因此，外切核酸酶A5是一种对核酸的糖不具有特异性的酶。当外切核酸酶A5用作核酸研究中的特异性试剂时，所报道的数据具有重要意义。