Gite S U, Shankar V
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
Crit Rev Microbiol. 1995;21(2):101-22. doi: 10.3109/10408419509113536.
Single-strand-specific nucleases, which act on single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids, are multifunctional enzymes and are ubiquitous in distribution. They find wide application as analytical tools in molecular biology research, although enzymes such as P1 nuclease are also used for production of flavor enhancers such as 5' IMP and 5' GMP. Because these enzymes are mainly used as analytical tools, very little attention was paid to aspects relating to their structure-function relationships. However, during the last few years considerable developments have taken place in this area. Single-strand-specific nucleases, their purification, characteristics, biological role, and applications have been reviewed.
单链特异性核酸酶作用于单链核酸以及双链核酸中的单链区域，是多功能酶，分布广泛。它们作为分析工具在分子生物学研究中得到广泛应用，尽管诸如P1核酸酶等酶也用于生产风味增强剂如5'-肌苷酸（5' IMP）和5'-鸟苷酸（5' GMP）。由于这些酶主要用作分析工具，所以很少关注与其结构-功能关系相关的方面。然而，在过去几年中该领域有了相当大的进展。单链特异性核酸酶、它们的纯化、特性、生物学作用及应用已得到综述。
