Tomioka C, Nishioka K, Kogure K
Department of Neurology, School of Medicine, Tohoku University, Sendai, Japan.
Brain Res. 1992 Jul 24;586(2):352-7. doi: 10.1016/0006-8993(92)91648-x.
We examined the changes in immunoreactivity of microtubuli-associated protein (MAP) 2 in dendrites by immunohistochemical analysis following 20 min of cerebral ischemia in the rat. A decrease of immunoreactivity of MAP 2 in dendrites in the CA1 subfield of the hippocampus was observed on days 3 and 7 but not on day 1 after ischemia. Early destructive changes of this protein were not observed, a finding which was confirmed by polyacrylamide gel electrophoresis analysis. We elucidated one factor which indicated that destruction of the dendrites of CA1 pyramidal neurons would not take place any earlier than the destruction of the neurons themselves after ischemia in rats.
我们通过免疫组织化学分析，研究了大鼠脑缺血20分钟后树突中微管相关蛋白（MAP）2免疫反应性的变化。在缺血后第3天和第7天，观察到海马CA1亚区树突中MAP 2免疫反应性降低，但在第1天未观察到。未观察到该蛋白的早期破坏变化，聚丙烯酰胺凝胶电泳分析证实了这一发现。我们阐明了一个因素，表明大鼠缺血后，CA1锥体神经元树突的破坏不会比神经元自身的破坏更早发生。
