Deschrevel B, Dugast J Y, Vincent J C
Laboratoire Polymères, Biopolymères, Membranes, U.R.A. n. 500 C.N.R.S., Université de Rouen, Mont-Saint-Aignan.
C R Acad Sci III. 1992;314(11):519-25.
The synthesis reaction of the peptide, N-Cbz-L-tryptophanyl-glycineamide, catalyzed by alpha-chymotrypsin was performed in a 20% water/80%, 1,4-butanediol mixture. The synthesis yield reached 90.9% at the end of the reaction and 72.3% after purification. The effects on the yield of both pH and the ratio between total initial concentrations of glycineamide and N-Cbz-L-tryptophan are examined. The high yield, specificity, simplicity and reproducibility of this method make it complementary of the chemical methods.
在20%水/80% 1,4 -丁二醇的混合物中，由α-胰凝乳蛋白酶催化进行肽N -苄氧羰基-L -色氨酰甘氨酰胺的合成反应。反应结束时合成产率达到90.9%，纯化后为72.3%。研究了pH值以及甘氨酰胺与N -苄氧羰基-L -色氨酸的总初始浓度之比对产率的影响。该方法的高产率、特异性、简便性和可重复性使其成为化学方法的补充。
