Hsieh Yu-Wen, Hwang Deng-Fwu
Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan, Republic of China.
J Agric Food Chem. 2004 Jun 30;52(13):4159-65. doi: 10.1021/jf035462l.
Phylogenetic relationships among puffer fish were investigated by comparing cytochrome b gene sequences and restriction endonuclease assays of 16 species from Taiwan. DNA was prepared for sequencing by PCR. No variation in sequences was detected among individuals within each species. Direct estimates of mitochondrial cytochrome b gene sequence divergence among 16 puffer fish were from 3.41 to 31.78%. Different restriction patterns were found among 16 puffer fish with 10 restriction endonucleases, whereas no variation in patterns was detected among individuals within each species. The polymorphisms obtained by RFLP have provided a new set of genetic markers for the accurate identification of sibling puffer species. It is the first molecularly based study of puffer diversity and sheds light on the evolution and taxonomy of this major puffer fish family.
通过比较台湾16种河豚的细胞色素b基因序列和限制性内切酶分析，研究了河豚之间的系统发育关系。通过PCR制备用于测序的DNA。在每个物种内的个体之间未检测到序列变异。16种河豚线粒体细胞色素b基因序列差异的直接估计值为3.41%至31.78%。用10种限制性内切酶在16种河豚中发现了不同的限制性模式，而在每个物种内的个体之间未检测到模式变异。通过RFLP获得的多态性为准确鉴定同胞河豚物种提供了一组新的遗传标记。这是第一项基于分子的河豚多样性研究，为这个主要河豚科的进化和分类学提供了线索。
