一种重组嵌合抗人绒毛膜促性腺激素抗体预防人细胞滋养层细胞融合及阻断孕酮合成的疗效。
Efficacy of a recombinant chimeric anti-hCG antibody to prevent human cytotrophoblasts fusion and block progesterone synthesis.
作者信息
Dhar Ruby, Karmakar Subhradip, Sriraman Rajan, Talwar Gursaran Prasad, Das Chandana
机构信息
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
出版信息
Am J Reprod Immunol. 2004 May;51(5):358-63. doi: 10.1111/j.1600-0897.2004.00169.x.
PROBLEM
A recombinant chimeric antibody against hCG (cPIPP) has been engineered and expressed at high yield in plants. The purpose of this work was to enquire whether this antibody is competent to neutralize the bioactivity of hCG on human trophoblasts.
METHODS
Cytotrophoblast cells, isolated from term placentae were maintained in culture for 3 days in presence or absence of humanized chimeric anti-hCG antibodies. Progesterone secreted was quantitated by ELISA. Fusion and cyto-architecture of the cells was studied by light and electron microscopy. Modulation of E-cadherin was investigated using RT-PCR and immunocytochemistry.
RESULTS
Recombinant chimeric anti-hCG antibody blocked the synthesis of progesterone by trophoblasts. No fusion of cytotrophoblasts to form syncytium took place. E-cadherin, a vital cell adhesion molecule involved in cell-to-cell interaction did not show differentiation related decline in its expression in presence of the antibody.
CONCLUSION
Recombinant chimeric anti-hCG antibody (cPIPP) was effective to neutralize hCG induced bioactivities in the human derived trophoblast cells.
问题
一种针对人绒毛膜促性腺激素（hCG）的重组嵌合抗体（cPIPP）已被构建并在植物中高产表达。这项工作的目的是探究该抗体是否能够中和hCG对人滋养层细胞的生物活性。
方法
从足月胎盘分离出的细胞滋养层细胞在有或无人源化嵌合抗hCG抗体的情况下培养3天。通过酶联免疫吸附测定法（ELISA）对分泌的孕酮进行定量。利用光学显微镜和电子显微镜研究细胞的融合及细胞结构。使用逆转录聚合酶链反应（RT-PCR）和免疫细胞化学研究E-钙黏蛋白的调节。
结果
重组嵌合抗hCG抗体阻断了滋养层细胞孕酮的合成。细胞滋养层细胞未发生融合形成合体滋养层。E-钙黏蛋白是一种参与细胞间相互作用的重要细胞黏附分子，在抗体存在的情况下其表达未出现与分化相关的下降。
结论
重组嵌合抗hCG抗体（cPIPP）可有效中和hCG在人源滋养层细胞中诱导的生物活性。
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