Saha Nil Ratan, Usami Takeshi, Suzuki Yuzuru
Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Maisaka 2971-4, Shizuoka 431-0211, Japan.
Fish Shellfish Immunol. 2004 Aug;17(2):149-58. doi: 10.1016/j.fsi.2004.01.001.
The effects of steroid hormones on in vitro IgM-secreting cells (IgMSC) and IgM secretion by lymphocytes of the lymphoid organs in common carp, Cyprinus carpio were examined by ELISPOT and ELISA assay, respectively. Cells isolated from peripheral blood (PB), spleen and head kidney were cultured for 12, 24 and 48 h either in the absence or in the presence of steroid hormones, i.e. cortisol, testosterone, 11-ketotestosterone (11-KT), and estradiol-17beta (E(2)) at doses of 1, 10 and 100 ng/ml. Cortisol reduced the IgMSC numbers and IgM secretion by cells from all organs. In addition, cortisol induced apoptosis in lymphocytes from all organs. High dose of testosterone showed tissue-specific functions; it reduced the number of IgMSC and amount of IgM secretion by cells from spleen and head kidney, but not in PB, though IgM secretion was suppressed. However, no effects of sex steroids were observed in this study. The results show that sex-specific steroid hormones may have no immunosuppressive effects in common carp.
分别通过酶联免疫斑点法（ELISPOT）和酶联免疫吸附测定法（ELISA）检测了类固醇激素对鲤（Cyprinus carpio）体外分泌IgM的细胞（IgMSC）以及淋巴器官淋巴细胞分泌IgM的影响。从外周血（PB）、脾脏和头肾分离出的细胞，在不存在或存在类固醇激素（即皮质醇、睾酮、11-酮睾酮（11-KT）和17β-雌二醇（E₂））的情况下培养12、24和48小时，类固醇激素的剂量分别为1、10和100 ng/ml。皮质醇减少了所有器官细胞的IgMSC数量和IgM分泌。此外，皮质醇诱导了所有器官淋巴细胞的凋亡。高剂量的睾酮表现出组织特异性功能；它减少了脾脏和头肾细胞的IgMSC数量和IgM分泌量，但外周血细胞的IgMSC数量和IgM分泌量未减少，不过IgM分泌受到了抑制。然而，在本研究中未观察到性类固醇的影响。结果表明，性别特异性类固醇激素可能对鲤没有免疫抑制作用。
